How to extract myofibroblasts from tissues?
Myofibroblasts are specialized cells involved in wound healing and tissue repair, but they can also contribute to fibrotic tissue formation. Isolating these cells from tissues requires a process of tissue digestion and cell separation techniques. The tissue is first mechanically minced and enzymatically digested to release the cells. Myofibroblasts can be identified by their phenotype using fluorescently labelled antibodies and sorted using flow cytometry. Additionally, they can be cultured in vitro for further characterization, and gene expression analysis can be carried out to understand their function in tissue repair and remodelling.
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